南农盖钧镒团队从野生豆中克隆控制粒重、蛋白含量、油分含量的基因

作者:莱肯生物 / 公众号:Lichen-Bio 发布时间:2019-07-11


一年生野生大豆籽粒具有低百粒重、高蛋白含量、低油分含量的表型性状,其所携带的控制这些性状的等位基因能够扩展栽培大豆的遗传资源。最近,南京农业大学盖钧镒团队利用野生大豆和栽培大豆构建的染色体代换系克隆到一个同时控制大豆百粒重、蛋白含量、油分含量的基因。
研究人员利用野生大豆N24852作为供体亲本,栽培大豆NN1138-2作为轮回亲本,构建了一个包含195个染色体代换系的群体。在该群体中,研究者检测到多个分别控制百粒重、蛋白含量、油分含量的染色体片段/QTL。再利用回交群体,研究者将一个控制3个性状的QTL定位在第15染色体329 kb的区域内,并将一个控制百粒重性状的QTL定位在第18染色体286 kb的区域内。结合转录组的数据发现,329 kb区域内的42个基因中只有Glyma.15g049200在两亲本种子发育的各阶段均有明显的表达差异。进一步分析该基因在野生大豆和栽培大豆中的基因组序列,发现野生大豆中该基因CDS区存在两个碱基的插入。该基因编码一个双向蔗糖转运蛋白,属于MtN3/saliva家族,该蛋白为3个性状的调控提供了共同的生化基础。
Theoretical and Applied Genetics, 06 July 2019
Identifying a wild allele conferring small seed size, high protein content and low oil content using chromosome segment substitution lines in soybean
Author
HongyanYang, WubinWang, QingyuanHe, ShihuaXiang, DongTian, TuanjieZhao, JunyiGai*
*: Nanjing Agricultural University, China
Abstract
Annual wild soybean characterized with small 100-seed weight (100SW), high protein content (PRC), low oil content (OIC) may contain favourable alleles for broadening the genetic base of cultivated soybeans. To evaluate these alleles, a population composed of 195 chromosome segment substitution lines (SojaCSSLP4), with wildN24852as donor and cultivatedNN1138-2as recurrent parent, was tested. InSojaCSSLP4, 10, 9 and 8 wild segments/QTL were detected for 100SW, PRC and OIC, respectively. Using a backcross-derived secondary population, one segment for the three traits (q100SW15,qPro15andqOil15) and one for 100SW (q100SW18.2) were fine-mapped into a 329-kb region on chromosome 15 and a 286-kb region on chromosome 18, respectively. Integrated with the transcription data in SoyBase, 42 genes were predicted in the 329-kb region whereGlyma.15g049200showed significant expression differences at all seed development stages. Furthermore, theGlyma.15g049200segments of the two parents were sequenced and compared, which showed two base insertions in CDS (coding sequence) in the wildN24852comparing to theNN1138-2. Since onlyGlyma.15g049200performed differential CDS between the two parents but related to the three traits,Glyma.15g049200was predicted a pleiotropic candidate gene for 100SW, PRC and OIC. The functional annotation ofGlyma.15g049200indicated a bidirectional sucrose transporter belonging to MtN3/saliva family which might be the reason that this gene provides a same biochemical basis for 100SW, PRC and OIC, therefore, is responsible for the three traits. This result may facilitate isolation of the specific gene and provide prerequisite for understanding the other two pleiotropic QTL.
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